DOI: 10.36871 / vet.san.hyg.ecol.201802010
UDC 619: 576.807.9
Authors
I. B. PAVLOVA
Institute of Veterinary Sanitation, Hygiene and Ecology - BRANCH of FSBI Federal Research Center of the Russian Academy of Science and Technology
E. M. LENCHENKO, G. S. TOLMACHEV
FSBEI HE "MOSCOW STATE UNIVERSITY OF FOOD PRODUCTION"
Abstract
The article presents the results of a study of the stages of formation of biofilms and sensitivity to antibacterial drugs of bacteria Pseudomonas aeruginosa. A technique has been developed for preparing preparations for research in an optical and scanning electron microscope, which allows one to consistently study bacterial populations of Pseudomonas aeruginosa at one object without disturbing the natural architectonics. We used cultures of gram-negative bacteria Pseudomonas aeruginosa No. 17 in the logarithmic growth phase. As antibacterial drugs, a solution of canned bile of cattle and pigs, antibiotics of various groups and disinfectants were used. To study the morphology of the P. aeruginosa population, the preparations were fixed in pairs of 25% glutaraldehyde (DV) solution. For staining, vapors of a 1% aqueous solution of osmic acid (OSO4) were used, dehydrated with vapors of propylene oxide. The effect of antibacterial drugs of various groups was studied in a population of P. aeruginosa. When studying the sensitivity of bacteria by diffusion into agar using standard commercial discs, it was found that P. aeruginosa are sensitive to the group of? -Lactam antibiotics, aminoglycosides, and quinolones. Experimental data on the influence of antibacterial drugs on the processes of biofilm formation expand the boundaries of knowledge in the field of studies of the adaptive capabilities of ubiquitous bacteria to long-term persistence both in a warm-blooded organism and in environmental objects.
Keywords
Pseudomonas aeruginosa, biofilms, population variability, optical microscopy, scanning electron microscopy.
