DOI: 10.36871 / vet.san.hyg.ecol.201802012
UDC 619: 614.31
Authors
N. A. SHURDUB, V. M. SOTNIKOV
Institute of Veterinary Sanitation, Hygiene and Ecology - BRANCH of FSBI Federal Research Center of the Russian Academy of Science and Technology
Abstract
The article presents the results of studies of sterilized milk for the presence of residual (total and free) ATP, the possibility of using sterile milk containing a significant amount of free ATP, as a complete universal energy substrate for the cultivation of E. coli. The level of ATP bioluminescence was determined using a HY-LiTE®2 luminometer (Merk, Germany) in accordance with the manufacturer's instructions using special test systems. It was found that during sterilization of milk, somatic and bacterial cells are destroyed, and only free ATP remains. The level of free ATP can indirectly judge the number of somatic cells in raw skim milk accepted by dairies and, as a result, the quality of milk produced. It was determined that when bacteria were introduced into sterilized milk after 6 hours of incubation, the level of free ATP decreased by 5 times, bacterial ATP increased by 4 times against the background of a 2-fold increase in the level of bacteria. At the same time, it was noted that in the MPA during this time period the ATP of bacteria increased only 1,3 times, in saline, the indicators remained at the same level.
Keywords
ATP bioluminescence, KMAFAnM, CFU, sterilized milk, E. coli.
