RELATIONSHIP BETWEEN THE NUMBER OF BACTERIAL ATP AND THE NUMBER OF MICRO-ORGANISM CELLS IN THE WASTE OF PURE AND MIXED CULTURES OF SCHERICHES AND GOLDEN STAFILOKOK

DOI: 10.36871 / vet.san.hyg.ecol.201802013
UDC 619: 614.31

Authors

V. M. SOTNIKOV, N. A. SHURDUB, D. V. GRUZNOV
Institute of Veterinary Sanitation, Hygiene and Ecology - BRANCH of FSBI Federal Research Center of the Russian Academy of Science and Technology

Abstract

The article presents the results of studies to determine the amount of bacterial ATP in pure and mixed cultures of microorganisms. The level of ATP bioluminescence was determined using a HY-LiTE®2 luminometer (Merk, Germany) in accordance with the manufacturer's instructions using special test systems. During the experiments, the numerical values ​​of bacterial ATP of E. coli and Staphylococcus aureus were determined. It was found that at a concentration of E. coli (1,5 ± 0,1) • 102 ... (2,1 ± 0,2) • 106 CFU / cm3, the ATP level was 323,9 ± 16,2 ... 22666,8 ± 906,7 , 1,4 RLU. With an equal number of bacteria, the level of ATP of S. aureus exceeded the level of ATP of Escherichia by 1,8 ... 423,4 times. It was noted that in mixed cultures of Escherichia coli and staphylococcus ATP indicators represented the average values ​​between ATP Escherichia and ATP of staphylococci and were in the range 29,6 ± 28334,1 ... 2550,1 ± 1,75 RLU with the number of microorganisms (0,1 ± 102 , 1,5) • 0,1 ... (106 ± 3) • XNUMX CFU / cmXNUMX. It was established that in pure and mixed cultures of microorganisms there is a linear relationship between the level of bacteria and ATP indices: the larger the number of bacteria, the higher the ATP level.

Keywords

ATP bioluminescence, CFU, KMAFAnM, Staphylococcus aureus, Escherichia coli.