DOI: 10.36871 / vet.san.hyg.ecol.201803009
UDC 619: 614.31
Authors
V. M. SOTNIKOV, N. A. SHURDUB, D. V. GRUZNOV, S. V. TOKAREV
FSBI "ALL-RUSSIAN RESEARCH INSTITUTE OF VETERINARY SANITARY, HYGIENE AND ECOLOGY"
Abstract
The paper reflects the results of determining the level of bacterial, free and total ATP in the secret of the udder of patients with mastitis cows. The level of ATP-bioluminescence, including indicators of total, free and bacterial ATP, was determined using a HY-LiTE®2 luminometer (Merk, Germany) and special test systems. When studying the secretion of the udder of cows with clinical purulent mastitis, it was impossible to determine the correlation between KMAFAnM and ATP values, since microorganisms were absent in 50% of the samples. It was established that in the secret of the udder of cows with a subclinical form of inflammation of the udder, the content of somatic cells was 2,5 ... 3 times higher than in milk of healthy animals. With an increase in the number of somatic cells, total ATP, in contrast to free, increased. At the maximum level of somatic cells, ATP indices differed 4 times. It was noted that with increasing values of total ATP in the secretion of the udder of sick cows, the content of microorganisms increased proportionally, but no correlation between KMAFAnM and the level of free ATP was noted. It was determined that in 14,3% of the samples of udder secretion at low somatic cells (at the level of indicators in healthy animals), an increased level of free ATP and KMAFAnM was observed. It was noted that with an increase in KMAFAnM, the values of ATP of microorganisms increase.
Keywords
ATP-bioluminescence, KMAFAnM, raw milk, mastitis of cows, dairy farms.
