FEATURES OF FORMATION OF MICROBIAL BIOFILMS UNDER THE INFLUENCE OF ELECTROCHEMICALLY ACTIVATED SOLUTIONS ON A POPULATION OF SOME PATHOGENIC BACTERIA

DOI: 10.36871 / vet.san.hyg.ecol.201804007
UDC 619: 579: 614.48

Authors

D. A. BANNIKOVA, A. B. KONONENKO
Institute of Veterinary Sanitation, Hygiene and Ecology - BRANCH of FSBI Federal Research Center of the Russian Academy of Science and Technology

Abstract

Field cultures of Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa were used as test cultures in the experiments. In this work, we used a technique based on the cultivation of bacteria in droplets of semi-liquid agar deposited on membrane filters, which allowed us not only to quantify the effect of ANCs on bacterial populations, but also to study the morphological changes occurring in bacterial populations. The use of scanning electron microscopy methods allowed us to state that the action of electrochemically activated solutions leads to partial destruction of the biofilms of bacterial colonies. Scattered bacterial cells with impaired cell wall integrity are deprived of the ability to adhere and subsequent colonization, which determines the initial stage of the occurrence of an infectious process in the body of a susceptible host.

Keywords

pathogenic bacteria, electrochemically activated solutions, bacterial biofilms, scanning electron microscopy.