DOI: 10.36871 / vet.san.hyg.ecol.201804008
UDC 619: 614.31

Authors

V. M. SOTNIKOV, N. A. SHURDUB, D. V. GRUZNOV, S. V. TOKAREV
Institute of Veterinary Sanitation, Hygiene and Ecology - BRANCH of FSBI Federal Research Center of the Russian Academy of Science and Technology

Abstract

The article presents the results of studies to determine the level of total, free and bacterial ATP in raw milk from healthy cows on a farm in the Moscow region. The amount of total ATP (bacterial, somatic and free), as well as the level of free ATP in raw milk samples were measured in accordance with the manufacturer's instructions using special test systems on a HY-LiTE®2 luminometer (Merk, Germany) after preliminary sample preparation. ATP of bacteria in milk was determined as the difference between total and free ATP after the destruction of somatic cells. It was noted that with an increase in the number of somatic cells in raw milk from healthy cows from 191,3 ± 9,6 to 310,4 ± 15,5 thousand / cm3, the level of total ATP increases from 10 389,8 ± 415,6 to 16 637,0, 499,1 ± 5274,8 RLU, free - from 210,9 ± 10 to 089,6 403,6 ± 2 RLU, while the total ATP is 2,1 times higher than the free ATP. It was found that the level of bacteria in raw milk from healthy animals ranged from (0,2 ± 104) • 1,9 to (0,1 ± 106) • 3 CFU / cm1,5, did not depend on the values ​​of somatic cells and did not correlate with indicators of total and free ATP. It was determined that with an increase in the number of microorganisms in raw milk, the values ​​of bacterial ATP increase proportionally. In this case, indicators can vary by 2 ... XNUMX times.

Keywords

ATP-bioluminescence, KMAFAnM, raw milk, healthy cows, dairy farms.