DOI: 10.36871 / vet.san.hyg.ecol.201904015
UDC 619: 614.31
Authors
SHURDUBA N.A., GRUZNOV D.V., TOKAREV S.V.
Institute of Veterinary Sanitation, Hygiene and Ecology - BRANCH of FSBI Federal Research Center of the Russian Academy of Science and Technology
Abstract
The article presents materials to determine the possibility of using ATP-bioluminescence to control the quality of the nipple rubber after washing and disinfection. The degree of ATP-bioluminescence, including indicators of total (free and bacterial) ATP, was determined using a HY-Lite®2 luminometer (Merk, Germany) and special test systems. It was found that on the inner surface of the nipple rubber, depending on the service life, KMAFAnM increases and, accordingly, ATP-bioluminescence increases. It was also noted that during the life of the nipple rubber a gray coating of milk stone forms, which irritates the skin of the nipples of the udder during milking and can disrupt its protective functions. Milk stone on nipple rubber promotes the formation of biofilms by pathogenic microorganisms that cause mastitis and impair the quality of raw milk. Measurement of ATP bioluminescence on the inner surface of the nipple rubber allows you to quickly monitor the effectiveness of washing and disinfection and timely take corrective measures in accordance with the principles of HACCP.
Keywords
ATP-bioluminescence, KMAFAnM, BGKP, enterobacteria, staphylococci, raw milk, nipple rubber, sanitation of the milking machine.