DOI: 10.36871 / vet.san.hyg.ecol.201904008
UDC 638.162: 669.018.674
Authors
SMIRNOV A.M., KLOCHKO R.T., LUGANSKY S.N., SOKHLIKOV A.B., BLINOV A.V., IGNATIEVA G.I.
Institute of Veterinary Sanitation, Hygiene and Ecology - BRANCH of FSBI Federal Research Center of the Russian Academy of Science and Technology
Abstract
The unregulated use of antibiotics for the treatment of infectious diseases of bees can lead to their accumulation in bee products, in particular in bee bread. The most effective methods for detecting residual amounts of antibiotics in bee bread is immunomicrochip technology, based on competitive chemiluminescent immunoassay and allowing semi-automated qualitative and quantitative analysis of a large number of samples. To determine the residual amounts of oxytetracycline in the bee bread, a test system with a Randox evidence investigator scanning chemiluminometer was used. The sensitivity of the method to the antibiotic oxytetracycline was 5,0 μg / kg with a confidence of 95%; the entire analysis together with sample preparation takes 3 ... 3,5 hours and does not require large expenditures of reagents. The method of immunomicrochip technology is highly sensitive and promising for the study of beekeeping products, in particular perga, for the presence of antibiotic residues.
Keywords
immunomicrochip technology, bee bread, antibiotics, tetracyclines.
