DOI: 10.36871 / VET.SAN.HYG.ECOL.202001010
УДК 579.67:579.85:615.9:593.174.1:576.08

Authors

KHABIROVA (GALLYAMOVA) S.R., MUKMINOV M.N.
KAZAN (VOLGA) FEDERAL UNIVERSITY, KAZAN, RUSSIAN FEDERATION
IDIYATOV I.I., BIRULYA V.V., SHURALEV E.A.
FEDERAL CENTER OF TOXICOLOGICAL, RADIATION AND BIOLOGICAL SAFETY, KAZAN, RUSSIAN FEDERATION

Abstract

The safety of strains of lactic acid bacteria Lactobacillus plantarum and Lactococcus lactis isolated from natural biotopes has been established. In an experiment on a kidney cell culture of a human embryo of the Hek 293 line, it was shown that the tested microorganisms do not adversely affect their morphology and proliferative activity, do not have cytotoxicity in titers of 3,9 ... 500 • 108 CFU / ml The values ​​of the studied parameters during co-cultivation with bacteria did not have significant differences from the control. The absolute number of cells in the experiment and control was in the range of 3,6 ... 4,3 • 105 cells / ml, the proliferation index was 0,68 ... 0,79, and the viability was 83,50 ... 89,90%. When exposed to Stylonychia mytilus ciliates, no toxic effect of the strains was revealed; the percentage of their death in the experimental and control samples did not differ significantly. The survival of protozoa after exposure to a cell suspension of Lactobacillus plantarum M3 in dilutions of 5 • 1011 ... 3,9 • 109 CFU / ml was 94,50 ... 92,67%, Lactobacillus plantarum M14 - 94,3 ... 93,50, Lactococcus lactis C4 - 94,33 ... 93,10, Lactococcus lactis C5 - 94,30 ... 93,20, Lactococcus lactis E8 - 94,67 ... 93,00%.

Keywords

lactic acid bacteria, cell culture, morphology, proliferative activity, protozoa, toxicity.