MORPHOLOGICAL FEATURES OF THE DEVELOPMENT OF BIOFILMS ON THE ABIOTIC SURFACES OF THE ENVIRONMENT

DOI: 10.36871 / VET.SAN.HYG.ECOL.202001008
UDC

Authors

BANNIKOVA D.A., KONONENKO A.B., PAVLOVA I.B., SUVOROV A.V.
Institute of Veterinary Sanitation, Hygiene and Ecology - BRANCH of FSBI Federal Research Center of the Russian Academy of Science and Technology

Abstract

The article presents material on the results of studying the formation of biofilms by microorganisms of various taxonomic groups on abiotic and biotic surfaces using SEM. The dependence of the severity of the biofilm on environmental factors (the nature of the surface, the temperature of the environment) is established. The cultures of the genera Salmonella, E. coli, Yersinia, Proteus, Klebsiella, Pseudomonas, Listeria, Staphylococcus were used in the work. To study morphology, 0,01 ml of suspension of bacterial test cultures with a concentration of 0,5 • 109 cells / ml was applied to sterile surfaces from various materials, placed in a moistened sterile Petri dish and kept at different temperature conditions for 72 ... 96 hours. natural architectonics, test objects were fixed in pairs of 25% glutaraldehyde for 3 ... 5 hours. To contrast the preparations, pairs of a 2 ... 4% solution of osmic acid were used for 2 ... 3 minutes. The obtained preparations after dehydration with propylene oxide vapor were examined in a HitachiTM 3030 scanning electron microscope (Japan). It was shown that in an aqueous medium, microorganisms are able to form structures similar to biofilms formed on dense nutrient media, however, these biofilms do not have a pronounced multilayer, characteristic of biofilms on dense substrates.

Keywords

conditionally pathogenic, pathogenic microorganisms, bacterial biofilm, scanning electron microscopy (SEM).