DOI: 10.36871 / vet.san.hyg.ecol.202003008
UDC 619: 579.262
Authors
Kononenko A.B., Bannikova D.A., Pavlova I.B.,
Britova S.V., Savinova E.P.
All-Russian Research Institute of Veterinary Sanitation, Hygiene and
Ecology – Branch of Federal State Budget Scientific Institution
«Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko
All-Russian Research Institute of Experimental
Veterinary Medicine, Russian Academy of Sciences»
Moscow, Russian Federation
Abstract
The article presents material on the results of experimental studies on the formation
of biofilms by microorganisms of individual taxonomic groups on various
surfaces of objects of the environment. The time and degree of biofilm formation,
as well as the dependence on the type of surface and bacteria, were established.
The cultures of the genera Salmonella, E. coli, Yersinia, Proteus, Pseudomonas
and Staphylococcus were used in the work. To study the formation of biofilms,
24-hour cultures of S-form microorganisms grown on solid or in liquid nutrient
media were used. Test objects were stained with 0,1% crystalline violet solution
for 15 minutes, unbound dye was washed off with physiological saline or distilled
water. Test objects were placed on filter paper and dried. Then 200 microliters of
96% ethanol were added to extract the paint from the film and the optical density
was measured on a KFK-3KM spectrophotometer at wavelength of 590 nm. It was
found that conditionally pathogenic and pathogenic microorganisms form a biofilm
on both smooth and rough abiotic surfaces within 48 hours. Moreover, the intensity
of biofilm formation on rough surfaces is higher than on smooth ones by 15...30%.
Among the studied microorganisms, the most intensive development of biofilm
was observed in Ps. cultures. aeruginosa and Pr. vulgaris. Biofilms pose a potential
danger of contamination of feed, food raw materials and, most importantly, food
products, since they can exist for a long time on a wide variety of surfaces of production
facilities of the respective enterprises.
Keywords
conditionally pathogenic, pathogenic microorganisms, bacterial biofilm, optical density, surfaces.