DOI: 10.36871 / vet.san.hyg.ecol.202004011
UDC 619: 579
Authors
1Kiseleva I.A., 1Nityaga I.M., 2Markova Y.M., 1Lutsay V.I.
1Moscow State University of Food Production,
Moscow 125080, Russian Federation
2Federal Research Centre of Nutrition and Biotechnology,
Moscow 109240, Russian Federation
Abstract
Products enriched with probiotic microorganisms have become increasingly popular in recent years. In this regard, it is particularly relevant to identify species and determine the safety of probiotic microorganisms in enriched products, which are aimed at identifying substandard products and preventing poisoning people, as well as confirming the compliance of the quantitative composition of probiotic microorganisms with regulatory documentation. The aim of the study is to develop a method for detecting probiotic cultures of mixed composition in enriched products by polymerase chain reaction. As a result, specific primers were selected for the following types of lactobacteries: L. acidophilus, L. casei, L. plantarum, L. fermentum. Quantitative identification of probiotic microorganisms in fortified foods has been performed.
Keywords
Bifidobacteries, lactobacteries, enriched products, PCR, speciesspecific primers.
