DOI: 10.36871 / vet.san.hyg.ecol.202004012
UDC 619: 579: 578.81

Authors

1Glazunov E.A., 2Zurabov F.M., 3Pavlova I.B., 3Tolmacheva G.S.
1Research and production center «MicroMir», Moscow 107031, Russian Federation
2Lomonosov Moscow State University, Moscow 119234, Russian Federation
3All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal State Budget Scientific Institution «Federal Scientific Center – K. I. Skryabin, Ya. R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences», Moscow 123022, Russian Federation

Abstract

One of the most important problems of our time is the growing level of resistance to antibiotics among human and animal pathogens. In this regard, more and more attention is paying to alternative therapeutic agents. In the present work, the effects of the bacteriophage vB_KpnP_FZXNUMX on Klebsiella pneumoniae biofilms have been investigated. Klebsiella pneumoniae XNUMX strain and the bacteriophage vB_KpnP_FZXNUMX were used. To study the formation of biofilms in liquid nutrient media by populations of Klebsiella pneumoniae XNUMX, XNUMX…XNUMX-hour cultures of microorganisms in the Sform (third generation) were used. A suspension of bacteria at a concentration of XNUMX×XNUMX CFU/ml in a volume of XNUMX ml was mixed on machine Vortex and introduced into Petri dishes with XNUMX ml of BHI broth. Sterile slides were placed on the bottom of a Petri dish, sterile fat-free cover glasses were placed on top, and incubated in a thermostat for XNUMX-XNUMX hours at XNUMX°C. After XNUMX hours, XNUMX ml of the bacteriophage vB_KpnP_FZXNUMX was added with a concentration of XNUMX×XNUMX PFU/ml, incubated in a thermostat for XNUMX hours at a temperature of XNUMX°C. In parallel, control glasses were incubated without adding phage. To preserve the natural architectonics, the samples were fixed in vivo in vapors of XNUMX% glutaraldehyde for XNUMX...XNUMX h. It was shown that the bacteriophage is able to effectively destroy the structure of biofilms and clusters, while reducing the total number of bacteria.

Keywords

bacteriophages, phages, biofilms, Klebsiella pneumoniae, antibiotic resistance.