DOI: 10.36871 / vet.san.hyg.ecol.202102017
UDC 619: 615.065: 615.37 / 281.9
Authors
Shabunin S.V.
Vostroilova G.A.
Gritsyuk V.A.
Chaplygina Yu.A.
Khokhlova N.A.
Federal State Budgetary Scientific Institution «All-Russian Veterinary Research
Institute of Pathology, Pharmacology and Therapy», Voronezh, Russian Federation
Abstract
The article presents the results of toxicometric assessment of Submastin-KRS in an acute experiment on white laboratory rats and mice when it is intragastrically and intramuscularly administered. The preparation was administered once into the stomach using a metal atraumatic probe at a dose range from 2000,0 to 27 mg/kg. A single injection volume was 500,0 ml (mouse) and 0,5 ml (rat); intramuscularly – at doses from 5,0 to 1200,0 mg/kg in a volume of 21 ml per mouse and 250,0 ml per rat. The animals of the Placebo group, administered the preparation in the same volume and by the same method of administration, received sterile isotonic sodium chloride solution. It was found that in case of the tested methods of administration in the range of the studied doses of the preparation, there was no deaths of animals, therefore, the mean lethal dose (LD0,25) of the preparation Submastin-KRS was not determined. Consequently, the experimental data obtained make it possible to classify the dosage form of the preparation Submastin-KRS to low-hazard substances.
Keywords
preclinical studies, Submastin-KRS, acute toxicity, white mice and rats, mean lethal dose (LD50), hazard classes.
