DOI: 10.36871 / vet.san.hyg.ecol.202103008
UDC 639.3.043.2

Authors

Pavlova E.V.
Putina T.G.
Moscow State University of Food Production Moscow, Russian Federation
Udavliev D.I.
Moscow State University of Food Production, Moscow, Russian Federation; All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal State Budget Scientific Institution «Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences» Moscow, Russian Federation

Abstract

The purpose of the work is a veterinary and sanitary examination of the quality and safety of feed for sturgeon fish, including the analysis of changes in the microflora of feed during storage in terms of organoleptic, physicochemical and microbiological indicators. Samples of fish meal, reworked loose fish meal, readymade fish food were taken for the study.
It has been established that 6 % of feed does not meet the regulatory requirements for organoleptic indicators, 3 % – for physicochemical indicators in excess of the mass fraction of crude ash by 57 % and the content of mass fraction of crude fat by 10 %. The content of MRL of heavy metals and toxicological indicators correspond to the regulatory requirements for all types of studied feed.
Microbiological indicators did not meet the regulatory requirements in 6 % of samples for the presence of sulfide-reducing anaerobes, microscopic molds in one sample of fish meal and the content of Alternaria ssp, Penicillium patulum, Aspergillus ssp and yeast Zygosaccaromyces ssp and Xeromyces ssp in one sample of ready-made fish.
At storing until the end of the shelf life, 94 % samples of fish meal from two manufacturers were benign in all respects, they could withstand storage for 6 months without signs of spoilage, 90 % of the samples of the finished fish food microbiological stability until the end of the shelf life and another 14 days after the expiration date declared by the manufacturer. At all stages of the study pathogenic bacteria: Salmonella ssp., enter pathogenic E. coli, L. monocytogenes, S aureus and other coagulase-positive staphylococci were absent in all studied feeds.
The species composition of fishmeal microflora changed during storage, the amount of mesophilic microflora decreased, the microflora of the feed was represented by saprophytic spore-forming microorganisms of the genera Bacillus ssp, including Bac. сereus 0,5×105 UFC/G and Clostridium ssp, psychrophilic bacteria of the genera Pseudomonas and Enterococcus. The greatest accumulation of microflora was observed on the 35th day of storage.

Keywords

feed for sturgeon, veterinary and sanitary examination, microorganisms, safety, storage, С. perfringens, mold.