UDC 619: 579
doi: 10.36871/vet.san.hyg.ecol.202104011
Authors
Ekaterina M. Lenchenko
Dmitriy V. Stepanov
Dmitriy A. Blumenkrants
Moscow State University of Food Production, Moscow, Russian Federation
Abstract
The results of studies general patterns formation heterogeneous structure biofilms gram-negative and gram-positive bacteria, as well as yeast-like fungi Candida spp. are presented. Рrocesses intercellular communication of various systematic groups microorganisms has common morphological and functional patterns biofilm formation. Heteromorphic structures of biofilms united by the intercellular matrix have been revealed in natural, industrial, and clinical conditions, both in the body of mammals and birds, and in food products, devices and equipment, animal husbandry and food production technologies. Indication in a large number of microcolonies, as well as yeast and micellar phases in isolates from pathological material of animals, was a differential sign in local and systemic pathologies. Under the influence drugs on biofilms microorganisms, a direct correlation was established between morphometric and densitometric indicators, reflecting a decrease in the frequency occurrence clusters and optical density, respectively. Under the bacteriostatic effect of chemotherapeutic and disinfecting drugs, accumulations altered cells of spheroplastic type, capable forming stable and unstable L-forms, were revealed. For detection of viable microorganisms in a heterogeneous population microorganisms in vitro and in vivo, fluorescence microscopy and culture media with growth factors for the repair cell wall of L-forms bacteria are promising.
Keywords
biofilms, microorganisms, optical density, adhesion, luminescence microscopy, spheroplasts, L-forms, destruction
