UDC 619: 578.835.1
DOI: 10.26155/vet.zoo.bio.202001003
Authors
R. Z. Nurgaziev
Doctor of Veterinary Sciences, Professor, Corresponding Member of the National Academy of Sciences of the Kyrgyz Republic, Rector of the Kyrgyz National Agrarian University named after K. I. Skryabin,
E.O. Abduraimov
Doctor of Biological Sciences, Deputy General Director for Science and Commercialization of the Research Institute of Biosafety Problems, town. Guards, Kazakhstan
A. R. Nurgazieva
Candidate of Biological Sciences, Head of the Laboratory of Virology and Biotechnology of the Kyrgyz Scientific Research Institute of Veterinary Medicine named after A. Duysheev,
E. D. Krutskaya
Candidate of Veterinary Sciences, Leading Specialist of the Laboratory of Virology and Biotechnology of the Kyrgyz Scientific Research Institute of Veterinary Medicine named after A. Duysheev
Abstract
This mesogenic strain of the Newcastle disease virus was isolated on the territory of the Kyrgyz Republic. During the cultivation of the Newcastle disease virus, the embryos were infected with virus-containing material according to the generally accepted technique into the allantoic cavity. From the studies it was found that when developing chicken embryos are infected with a BN virus strain with a biological activity of at least 8,8 lg EID50 / cm3, any methods of virus dilution can be used with a volume of introduced material of 0,2 cm3 and virus dilution with an inoculum volume of 0,1 cm3. But at the same time, it is practically unsuitable for these purposes, since it contains an increased amount of protein, an admixture of blood cells and the contents of the yolk sac. After conducting several series of experiments and analyzing all the factors in order to prepare an inactivated vaccine from developing chicken embryos, we established the most optimal age for chicken embryos to cultivate the selected strain — 12–13 days.
Keywords
vaccine, mesogenic strain, allantoic fluid, chicken embryos.
