Improving the method of helminthological autopsy of the liver with maral dicroceliosis

UDC 636.294: 576.89: 608.2
DOI: 10.26155/vet.zoo.bio.202003004

Authors

M. Yu. Tishkov
Candidate of Veterinary Sciences, Leading Researcher, Federal State Budgetary Institution of Higher Vocational Education of the Russian Academy of Science, Department of VNIIPO,
O. N. Shmakova
Researcher, Laboratory for Animal Breeding and Animal Diseases, Federal Altai Scientific Center for Agrobiotechnology

Abstract

In the experiment, we compared the classical method of complete helminthological autopsy of the liver and the method of liver research we developed to determine the intensity of invasion of maral dicroceliosis. For this, 16 organs from animals of different ages were used. The organs were cut into three equal parts across the bile ducts, each of which was cut into plates: the first part 3–5 mm, the second part 6–8 mm and the third part 9–12 mm. As a result, it was found that plates with a thickness of 3-5 mm are difficult to evenly cut, but the efficiency of one washing is 97,4%, and the number of unwashed parasites is 2,5%. The minimum plate thickness affects the maximum number of damaged helminths 62,8%, intact - 34,7%. The cut thickness of 6–8 mm makes it possible to wash up to 95,0% of parasites in one wash with a small amount of damaged dicrocelium during cutting - 12,9%, the number of intact parasites reaches 83,5%, and unwashed helminths 3,6%. Plates 9-12 mm are easily cut, but poorly washed the first time, the efficiency is 85,5%, the number of unwashed parasites reaches 14,5%. Damaged diccelium during slicing only 4,1%, and intact - 81,4%. Improvement of the method of complete helminthological autopsy of the liver allows one to identify from one flushing from 85,5 to 97,4% of parasites from their total number. The liver examination time is reduced by 2-3 times compared with the classical technique, which, in one (first) rinse, does not give a clear picture of the intensity of invasion.

Keywords

laboratory equipment, maral liver, helminthological autopsy, dicrocelia, liver washing, liver section thickness, number of washes, invasion intensity.