DOI: 10.36871/vet.zoo.bio.202004004
UDC 579.63
Authors
Le Thi Ngoc An’
Post-graduate student of the Department of Microbiology,
Moscow State Academy of Veterinary Medicine
and Biotechnology – MVA by K. I. Skryabin,
Moscow, Russian Federation
T. N. Gryazneva
Doctor of biological Sciences, Professor, head of the Department
of Microbiology, Moscow State Academy of Veterinary
Medicine and Biotechnology – MVA by K. I. Skryabin,
Moscow, Russian Federation
Nguyen Ngoc Hai
Professor at the University of agriculture and forestry,
Ho Chi Minh city, Vietnam
Abstract
Reducing the level of aflatoxin contamination of animal feed using soil-isolated cultures of B. subtilis, showed the prospects of using this type of bacteria for decontamination of feed. A total of 367 B. subtilis cultures were isolated from soil in southern Vietnam and screened for inhibition of aflatoxin production by Aspergillus in vitro. Of these, 34 isolates of biologically active B. subtilis were selected, of which 5 isolates were the most resistant to aflatoxin. These cultures of bacilli after 26,76 days of cultivation in a mixture with Aspergillus on crushed corn contributed to a XNUMX-fold decrease in aflatoxin levels compared to the control. The data obtained indicate that B. subtilis isolates isolated from soil can inhibit aflatoxin in vitro.
Keywords
B. subtilis, aflatoxin, liquid medium, coconut oil agar.
