DOI: 10.26155/vet.zoo.bio.202005001
UDC 619: 615.038: 616-085

Authors

A. А. Lavrik
General Director, NoviStem, LLC, Belgorod, Russian Federation
S. G. Ali
Deputy General Director, NoviStem, LLC, Belgorod, Russian Federation
V. B. Moskalev
NoviStem, LLC, Belgorod, Russian Federation
O. A. Nikolchenko
Candidate of Biological Sciences, Senior Researcher of the Department of Experimental Modeling and Transplantology with the Experimental Biological Clinic, Sytenko Institute of Spine and Joint Pathology of National Ukrainian Academy of Medical Sciences, Kharkiv, Ukraine
F. S. Leontieva
Candidate of Biological Sciences, Head of the Department of Laboratory Diagnostics and Immunology, Sytenko Institute of Spine and Joint Pathology of National Ukrainian Academy of Medical Sciences, Kharkiv, Ukraine
N. A. Ashukina
Candidate of Biological Sciences, Head of the Laboratory of Connective Tissue Morphology, Sytenko Institute of Spine and Joint Pathology of National Ukrainian Academy of Medical Sciences, Kharkiv, Ukraine

Abstract

The regenerative properties of the «UltraCell-Dog» drug, obtained from the dog mesenchymal stem cell secretomе, were studied during early stages of the reparative process after modeling injury in rats. It was shown that the «UltraCell-Dog» stimulates the angiogenesis in the defect zone, promotes the formation of bone and cartilage tissue without structural disturbances. The data of biochemical analysis indicate a higher intensity of cartilage and bone tissue repair in the experimental group at the early stages of therapy. The regenerative properties of the «UltraCell-Dog» low-molecular (up to 50 kDa) peptide drug obtained from the secretion of dog mesenchymal stem cells were studied using a model of traumatic knee injury. It was shown histologically that «UltraCell-Dog» promotes the formation of bone and cartilage tissue without structural disturbances, and stimulates angiogenesis in the defect zone in the early stages of the reparative process. Biochemical assay data indicate a higher rate of recovery of cartilage and bone tissues in the experimental group comparing to the control.

Keywords

knee injury, veterinary science, stem cell secretomе, «UltraCell-Dog» drug, histology, blood serum biochemistry.