DOI: 10.26155/vet.zoo.bio.202005004
UDC 579.63
Authors
Le Thi Ngoc An’
Post-graduate student of the Department of Microbiology,
Moscow State Academy of Veterinary Medicine
and Biotechnology – MVA by K. I. Skryabin,
Moscow, Russian Federation
T. N. Gryazneva
Doctor of Biological Sciences, Professor, Head of the Department
of Microbiology, Moscow State Academy of Veterinary Medicine
and Biotechnology – MVA named after K. I. Skriabin,
Moscow, Russian Federation
Nguyen Ngoc Hai
Professor at the University of Agriculture and Forestry,
Ho Chi Minh city, Vietnam
Abstract
Soil-isolated B. subtilis bacilli were tested by PCR for the content of the target ituD and lpa- 14 genes and determined the ability of the bacilli to inhibit aflatoxins in crushed corn grain. B. subtilis containing either the ituD gene or the lpa-14 gene, as well as B. subtilis that do not contain these genes and other types of bacteria with the ituD and lpa-14 genes were used as controls. It was found that the presence of two target ituD and lpa-14 genes in B. subtilis significantly increased the ability of bacilli to inhibit aflatoxins. In comparison with the control, the reduction of aflatoxin in corn grain by bacilli of the strains B. subtilis No. 8 and B. subtilis No. 9 was 26,76 and 14,45 times, respectively.
Keywords
B. subtilis, aflatoxin, ituD gene, lpa-14 gene, corn grain.
