Influence of sterilizing agents on callus formation of explants of medicinal plants (Dioscorea caucasia Lypsky.) with a high content of phenolic compounds under in vitro conditions

DOI: 10.26155/vet.zoo.bio.202005007
UDC 633.88

Authors

E. A. Kalashnikova
Doctor of Biological Sciences, Professor of the Department of Genetics, Breeding and Biotechnology, Timiryazev State Agrarian University of the Moscow Agricultural Academy, Moscow, Russian Federation
S. M. Zaytseva
Candidate of Biological Sciences, Associate Professor of the Department of Feeding and Feed Production, Moscow State Academy of Veterinary Medicine and Biotechnologies – MVA by K. I. Skriabin, Moscow, Russian Federation
Doan Thu Thuy
Candidate of Biological Sciences, Associate Professor of the Faculty of Agronomy, Vietnam National University of Agriculture, Hanoi, Vietnam
R. M. Kirakosyan
Candidate of Biological Sciences, Associate Professor of the Department of Genetics, Breeding and Biotechnology, Timiryazev State Agrarian University of the Moscow Agricultural Academy, Moscow, Russian Federation

Abstract

We studied the effect of various sterilizing agents on the viability of explants and the production of a productive culture in vitro Dioscorea, as well as the role of polyphenols on these processes. It is shown that during the initiation of calluses on explants, polyphenols are released into the nutrient medium, which further leads to inhibition of growth processes. The resulting callus cultures were characterized by a high content of polyphenols.

Keywords

polyphenols, in vitro.