Use of sugar in nutrition of microorganisms of Medusomyces gisevii at various concentrations of plant extracts in cultural liquid

DOI: 10.26155/vet.zoo.bio.202006011
UDC 543.068.8: 615.077

Authors

V. V. Rogozhin
Doctor of Biological Sciences, Professor of the Department of Agronomy and Chemistry, Yakut State Agricultural Academy, Yakutsk, Russian Federation
T. V. Rogozhina
Candidate of Biological Sciences, Associate Professor of the Department of Humanitarian and Legal Disciplines, East-Siberian Institute of Economics and Management, Yakutsk, Russian Federation

Abstract

The mechanisms of the regulatory action of various concentrations and the nature of the extracts of teas (black and green) and coffee on the productivity of Medusomyces gisevii, using sugar as a source of carbohydrates, were studied. In experiments, an artificial nutrient medium with a volume of 0,2 l was used, with a content of 10% inoculum. As a nutrient substrate in the culture medium of the microorganisms Medusomyces gisevii, sugar was added at a concentration of 100 g/l and the concentration of extracts of black or green tea or coffee varied from 0 to 18 g/l in the culture liquid. It was found that the action of the concentrations of tea and coffee extracts contains a complex regulatory mechanism that allows the substances contained in the plant extract to influence the activity of enzymes and their ability to catalyze the course of various chemical reactions in symbiont cells. It is shown that the blocking of the mechanisms of the action of enzymes causes a disruption in the functioning of the existing system of mutual complementarity and synchronism of the course of metabolic processes in the symbiotic community. At the same time, the established sequence in the metabolism of nutrient substrates between yeast and acetic acid bacteria is disturbed. As a result, the symbionte is forced to enter a state of forced rest, while maintaining viability, but losing the productivity of metabolic systems.

Keywords

Medusomyces gisevii, quorum sensing, culture media, sugar, sucrose, glucose, fructose, symbiotic communities, zoogloea, bacterial cellulose, microorganisms, electrical conductivity, acidity of the medium, black tea, green tea, coffee.