DOI: 10.26155/vet.zoo.bio.202007010
UDC 543.068.8: 615.077
Authors
V. V. Rogozhin
Doctor of Biological Sciences, Professor of the Department of Agronomy
and Chemistry, Yakut State Agricultural Academy,
Yakutsk, Russian Federation
T. V. Rogozhina
Candidate of Biological Sciences, Associate Professor of the Department
of Humanitarian and Legal Disciplines, East-Siberian
Institute of Economics and Management,
Yakutsk, Russian Federation
Abstract
The role of ascorbic acid (AK) in the productivity of Medusomyces gisevii extracts of black tea with sugar or glucose was studied. It is shown that the presence of AK in the culture fluid causes a decrease in pH values and contributes to an increase in the electrical conductivity, whose values correlate with an increase in the concentration of vitamin in the medium. It has been found that low concentrations of AK (0,25–1,0 g/l) show little effect of activation of the metabolic systems Medusomyces gisevii, while high concentrations of vitamin (10,0–50,0 g/l) have an inhibitory effect on symbiont microorganisms in a culture liquid with sugar or glucose. At the same time, high concentrations of AK (25,0–50,0 g/l) in the culture liquid Medusomyces gisevii with glucose completely suppress the activity of symbionts involved in the synthesis of bacterial cellulose. It was revealed that when using glucose as a nutrient substrate, the symbiont synthesizes biocellulose, whose dry mass is 3,8–5,7 times less than when using sugar. Therefore, the use of sugar as a nutrient substrate Medusomyces gisevii allows to obtain bacterial cellulose of higher quality, greater elasticity and strength, which can find application in medicine and cosmetology.
Keywords
Medusomyces gisevii, quorum sensing, culture media, symbiotic communities, zoogloea, sugar, glucose, bacterial cellulose, microorganisms, electrical conductivity, acidity of the medium, ascorbic acid.
