DOI: 10.36871/vet.zoo.bio.202107010
УДК 636.2.034+612.12:577.16+612.661.5:577.16
Authors
A. A. Savina
Junior Researcher, Department of Physiology and Biochemistry
of Farm Animals, All-Russia Research Institute of Animal
Husbandry named after Academy Member L. K. Ernst,
Dubrovitsy, Russian Federation
O. A. Voronina
Candidate of Biological Sciences, Veterinary Doctor, Senior Researcher
of the Department of Physiology and Biochemistry, All-Russia
Research Institute of Animal Husbandry
named after Academy Member L. K. Ernst,
Dubrovitsy, Russian Federation
S. Yu. Zaytsev
Doctor of Biological Sciences, Doctor of Chemistry, Professor, Leading
Researcher at the Department of Physiology and Biochemistry,
All-Russia Research Institute of Animal Husbandry
named after Academy Member L. K. Ernst,
Dubrovitsy, Russian Federation
Abstract
The aim of the work was to study the main biochemical parameters, including the measurements
of the total amount of water-soluble antioxidants by the amperometric method, milk
samples and the number of somatic cells in the milk of black-and-white cows in the summer period
of animal keeping.
In the work, the total amount of water-soluble antioxidants (TAWSA) was assessed by the amperometric
method (on the TsvetYauza 01-AA device) and the average values of the component composition
of milk for two groups of cows, which were formed by the number of somatic cells (NSC). Milk of mastitis cows causes food poisoning, that is why two groups were formed according to the
NSC values: 100) less than 100 thousand units/ml; 700) from XNUMX to XNUMX thousand units/ml, in milk samples
of black-and-white cows of the Moscow region that was used as raw material.
The TAWSA values for 11 samples of milk from cows in group 1 ranged from 20,9 mg/g to
41,4 mg/g (average value 27,8±5,1 mg/g); mass fraction of fat (MFF) – 4,95±0,80%; protein mass
fraction (PMF) – 3,13±0,27%; milk yield is about 20,5±4,1 L/day. Found correlations between
TAWSA and the following parameters of milk: MFF – –0,25; PMF – +0,30; SOMO – –0,39; milk
yield – +0,146.
The TAWSA values for 16 milk samples of cows in group 2 ranged from 18,8 mg/g to 29,5 mg/g (average
value 23,6±2,1 mg/g); MFF – 4,77±0,39%; PMF – 3,36±0,22%; milk yield about 20,0±3,1 L/ day.
Found correlations between TAWSA and the following parameters of milk: MFF – –0,37; PMF –
+0,11; SOMO – –0,32; milk yield – –0,12.
The data obtained are of great importance both in assessing the physiological and biochemical
status and the state of the antioxidant defense system of the cow’s organism.
Keywords
analytical biochemistry, antioxidant protection, somatic milk cells, composition of cow’s milk, amperometric detection.