UDC 579.62
DOI: 10.36871/vet.zoo.bio.202110006
Authors
Seidfatima M. Borunova
All-Russian State Center for Quality and Standardization of Medicinal
Products for Animals and Feed, Moscow, Russia; Moscow State Academy of Veterinary Medicine and Biotechnology –
MBA named after K. I. Scriabin, Moscow, Russia
Olga V. Karabanova
Ochir E. Badmaev
Anastasia V. Pomazkova
Maria A. Egoreva
All-Russian State Center for Quality and Standardization of Medicinal
Products for Animals and Feed, Moscow, Russia
Abstract
The research is focused on the standardization of nutrient media while using the microbiological
method of veterinary and sanitary control of the quality of dog sperm for the presence of pathogenic
microorganisms, namely, coliform bacteria.
25 samples of frozen sperm of sire males were examined for a microbiological indicator (determination
of bacteria coliform group). The samples were provided by the clinical diagnostic center FGBU
«VGNKI». During the research a comparative test of Bulir's nutrient medium was carried out. Also
were carried out tests for other nutrient media recommended for isolating coliform bacteria.
Keywords
canine sperm, microbiology, Escherichia coli, nutrient media, coli-titer, validation of the microbiological method
