UDC 619.616.98.579.841.935.07
DOI: 10.36871/vet.zoo.bio.202205003
Authors
Svetlana V. Zyuzgina,
Olga E. Zinovieva,
Central Scientific and Methodological Veterinary Laboratory, Moscow, Russia
Saida N. Marzanova,
Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K. I. Skryabin”, Moscow, Russia
Abstract
For the serological diagnosis of brucellosis in cattle in veterinary laboratories of the Russian
Federation, one or several tests are used simultaneously. The sensitivity and specificity of the
determination of antibodies to the pathogens of this disease using various methods varies significantly,
none of the serological tests is suitable for all stages of the infectious process in brucellosis.
ELISA is a modern serological test that has not found wide application in the state veterinary
laboratories of the Russian Federation, although the number of studies conducted by this method
increases annually.
The use of enzyme-linked immunosorbent assay makes it possible to confirm the diagnosis of
brucellosis at all stages of the infectious process and to identify more seropositive animals than
classical serological methods.
Among serological methods, enzyme-linked immunosorbent assay (hereinafter referred to as
ELISA) is the most sensitive, specific and easy-to-perform test. In accordance with the Order of
the Ministry of Agriculture of the Russian Federation dated 08.09.2020 No. 533 «On approval of
Veterinary rules for the implementation of preventive, diagnostic, restrictive and other measures,
the establishment and cancellation of quarantine and other restrictions aimed at preventing the
spread and elimination of foci of brucellosis (including infectious epididymitis of sheep)» the ELISA
method is one of the confirmatory in the diagnosis of brucellosis. The data obtained as a result of
the studies indicates that the results of ELISA coincide with the complex of classical serological
methods (BRS – Bengal roses sample; AR – agglutination reaction; CFR – complement fixation
reaction; RIH – reaction of indirect hemaggtination; IDR – immunodiffusion reaction) and a higher
diagnostic sensitivity of the test.
Keywords
brucellosis, antibodies, enzyme immunoassay, specificity, sensitivity