Subpopulation analysis of sheep blood lymphocytes by immunocytochemistry methods

UDC 619: 616.34-002.153: 636.7
DOI: 10.36871/vet.zoo.bio.202207002

Authors

Zevar Kh. Abdurakhmonova,
Kristina Yu. Permyakova,
Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K. I. Skryabin”, Moscow, Russia
Irina Yu. Ezdakova,
Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after K. I. Scriabin and Ya. R. Kovalenko of the Russian Academy of Sciences, Moscow, Russian Federation

Abstract

Due to the introduction of monoclonal antibodies into immunodiagnostics, it became possible to expand the methods of assessing post-vaccination immunity, namely, determining the number and functional activity of cells of the immune system by immunocytochemistry methods.
In this work, the quantitative characteristics of T- and B-lymphocytes, NK cells of sheep by immunoperoxidase staining and the number of SIGMA cells in the process of post-vaccination immune response by indirect immunofluorescence were determined.

Keywords

T- and B-lymphocytes, NK cells, monoclonal antibodies, immunodiagnostics, postvaccination immunity.