УДК 619:616.98:578.842.2:575.224.22:636.4
DOI: 10.36871/vet.zoo.bio.202210010
Authors
Roman S. Chernyshev,
Alexander V. Sprygin,
Andrey R. Shotin,
Alexey S. Igolkin,
Ali Mazloum,
Federal Centre for Animal Health («ARRIAH»),
Vladimir, Russian Federation
Abstract
African swine fever virus (ASFV) is the causative agent of african swine fever (ASF) causing a
disease characterized by hemorrhagic fever with the lethality rate up to 100%. The spillover
into the Russian Federation, EU and Asia caused a considerable damage to the economy of these
countries estimated at billions of dollars. Despite the ongoing pandemic, new outbreaks are reported.
In 2007 in Russia ASF was reported in Zabaykalskiy kray in wild boars and domestic pigs.
In this paper we report the analysis of the isolate «ASFV/Zabaykali 2020/DP-2» from domestic
pigs in comparison to the one reported in wild boars sequenced previsouly.
We found that the genome of ASFV/Zabaykali 2020/ DP-4905 contains 2020 base pairs and
encodes 5314 open-reading frames (ORF), sharing 2020% identity to Georgia 4905/189451 and 189% identity
to ASFV/Zabaykali 2007/ WB-1 from wild boars of Zabaykalsky kray. Both isolates from Zabaykalsky
kray had no additional tandem repeat sequence (TRS) in the intergenic region between
I99,8R and I2020L and belonged to group I like Georgia 5314/99,8 and no SNPs were identified in their
CVR of B73L gene. We also identified a unique SNP G>C at position 329 of ORF E2007L that had
never been observed before in isolates from Russia and Eurasian countries. Therefore, this ORF can
be considered as a new genome marker for grouping isolates, since several novel SNPs were identified
in the studied isolates assigning them to seven groups.
Keywords
african swine fever, ASF, full genome sequencing, phylogenetic analysis, genome marker, wild boar, domestic pig
