UDC 57: 579: 579.6: 579.62
DOI: 10.36871/vet.zoo.bio.202210005

Authors

Vladimir V. Ermakov,
Galina V. Molyanova,
Samara State Agrarian University, Samara, Russia

Abstract

The study was conducted with the aim of restoring the obligate and facultative resident microflora of the gastrointestinal tract of raccoons by using the experimental synbiotic Probat C-1. The study involved male and female raccoons of the control and experimental groups. Modeling the development of escherichiosis by intraperitoneal administration of Escherichia coli M 17 to raccoons led to a pronounced reaction of natural resistance and the immune system of the animal organism. The number of leukocytes, including lymphocytes and segmented neutrophils, increased in the blood. Among lymphocytes, the number of T-lymphocytes, B-lymphocytes increased, the concentration of gamma globulins increased. The number of leukocytes in the blood of raccoons of the experimental group increased from 9,86 (109/l) on the first day to 1 (14,32/l) on the third day of the study. At the same time, the number of Escherichia coli in the microbiocenosis of the gastrointestinal tract of raccoons increased significantly, while the number of enterococci, bifidobacteria, lactobacilli and bacilli decreased. The number of Escherichia coli in raccoons of the experimental group increased from the first day from 109×3 to 1×6,18 on the third day of the study. As a result, the colonization resistance of the resident microflora was significantly impaired, Escherichia coli increased its antilysozyme and anticarnosine activity, and its ability to form biofilms decreased. The use of the synbiotic «Probat C-106» in this situation helps to increase the potential of natural resistance and the immune system of the animal organism. At the same time, the number and colonization resistance of the obligate resident microflora are restored. This allows the resident microflora to fully manifest its biological properties, in particular antagonism towards transient pathogenic and opportunistic microflora.

Keywords

raccoons, microflora, synbiotic, escherichiosis, Escherichia coli