UDC 616.98: 619: 579.62
DOI: 10.36871/vet.zoo.bio.202211004
Authors
Olga E. Zinovieva,
Svetlana V. Zyuzgina,
Testing Central Scientific and Methodological Veterinary Laboratory
of Federal Center for Animal Health Protection,
Moscow, Russian Federation
Vasiliy I. Belousov,
Testing Central Scientific and Methodological Veterinary Laboratory
of Federal Center for Animal Health Protection,
Moscow, Russian Federation; Moscow State Academy of Veterinary Medicine and Biotechnology –
MVA by K. I. Skryabin, Moscow, Russian Federation
Abstract
Leptospirosis is a dangerous zoonotic disease caused by pathogenic bacteria Spirochaetales of
the genus Leptospira. To detect antibodies against Leptospira hardjo in the blood serum from
50 heads of vaccinated cattle and 40 heads of unvaccinated cattle, a comparative study was conducted
using the methods of МАТ, ELISA and IHA.
During the study of unvaccinated cattle, the coincidence between serological methods was 95 %,
the tests are identical in specificity and sensitivity.
Among vaccinated cattle in the MAТ with Leptospira Hardjo, 70 % of positive results were obtained,
58 % in IHA and 42 % in ELISA, a suspicious result was obtained in 4 % in ELISA and 9,5%
in IHA. Suspicious animals in ELISA and IHA reacted in MAТ no higher than titer 1/100.
Any of the presented methods of serological diagnostics is suitable for the control of this pathogen.
But for a correct diagnosis, the history of vaccination of animals should be taken into account.
Keywords
leptospirosis, antibodies, microagglutination reaction, enzyme immunoassay, immunochromatographic analysis
