Features of obtaining in vitro cultures of relict medicinal plants with a high content of biologically active substances (Taxus canadensis Marsh, Ginkgo biloba, Sequoia sempervirens L., Dioscorea caucasia Lypsky)

UDC 574.577
DOI: 10.36871/vet.zoo.bio.202305009

Authors

Svetlana M. Zaytseva,
Elena A. Kalashnikova,
Rima N. Kirakosyan,
Elisaveta A. Bolotina,
Sergey A. Dubovoy,
Sofiko O. Didmanidzhe,
Jing Liang,
State Agrarian University – Moscow State Agricultural Academy named after K. A. Timiryazev, Moscow, Russia

Abstract

The effect of the biochemical status of plants on the viability of explants and obtaining a productive culture of medicinal plants in vitro, taking into account the use of various sterilizing agents and photoperiod, was studied. It was found that the applied lighting mode had a significant effect on the intensity of callus tissue formation, its consistency and color. As a rule, callus tissue was formed in those places where the localization of phenolic compounds was insignificant. When calluses are initiated on explants characterized by a high content of polyphenols, they are released into the nutrient medium, which further leads to inhibition of growth processes. The resulting callus cultures were characterized by a high content of polyphenols, which were localized in cell walls and vacuoles.

Keywords

phenolic compounds, in vitro, localization, dioscorea, yew, double-lobed ginkgo, Sequoia