UDC 636.2.034 + 636.08
DOI: 10.36871/vet.zoo.bio.202306015

Authors

Sergei Y. Zaitsev,
Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst, Dubrovitsy, Russia

Abstract

The amino acid composition of gelatins, as well as their other properties, varies significantly depending on the sources and types of initial collagens and a number of technological conditions for their production. On the other hand, the amino acid composition of gelatins is one of the important indicators characterizing the quality of these intermediates, which consist of a number of peptide substances. According to the author, gelatin is a polypeptide product of the hydrolysis of the original protein – animal collagen. This complex definition is a combination of a number of known gelatin definitions. The need for this review is due to the fact that there are numerous, but not systematized, data on the content of amino acids in gelatin isolated from various tissues of cattle (large horn cattle), such as hide, bones, etc. To determine the amino acid composition of biological samples, various methods are used, one of which is widely used in scientific research, the method of high performance liquid chromatography in a variety of ion-exchange chromatography.
It is well known that gelatins are obtained from the collagen of a number of animals after treatment for about a day with various acids or alkalis (with possible hydrolysis by enzymes) in a cyclic technological process. This review critically discusses the results of pre-treatment of starting materials with hydrochloric, acetic, citric acid (for 21–26 hours) with further extraction under various conditions and features of the amino acid composition of the resulting gelatins. Changes in the amino acid composition of gelatins after the treatment of bovine collagen with various preparations under specified conditions are discussed in detail. The scientific and practical significance of this review lies in a detailed description of the main studies on the amino acid composition of gelatins and the identification of correlations between various biochemical and technological indicators of these products.

Keywords

animal biochemistry, cattle, proteins, gelatins and collagens, amino acid composition