UDC 636.5.087.69
doi: 10.36871/vet.san.hyg.ecol.202104012
Authors
Inna B. Pavlova
Anna Yu. Arsenyuk
Daria A. Bannikova
Dmitry V. Gruznov
Ekaterina P. Savinova
All-Russian Research Institute for Veterinary Sanitation, Hygiene and Ecology –
Branch of Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko
All-Russian Research Institute of Experimental Veterinary Medicine,
Russian Academy of Sciences», Moscow, Russian
Federation
Abstract
The article presents the results of electron microscopic investigations of populations of pathogenic Candida albicans № 138 from the museum collection. The morphology of the fungus, its ultrastructure and phases of development in the population were studied. The methods developed at VNIIVSGE for growing bacteria and fungi on the surfaces of Millipore membrane filters without disturbing the architectonics of populations were used in research. The preparations were examined with electron microscope Hitachi 800 (Japan) with two systems: transmission and scanning. At large magnifications, the features of the ultrastructure of Candida albicans cells were studied. It was found that in addition to budding, Candida albicans has a survival strategy in the form of the formation of small blastospore cells, the structure of which is visible only with the use of a scanning electron microscope. The forms, sizes and localization of blastospores with their own development cycle have been studied. The morphology of pseudogyphs, which play an important role in the existence and survival of fungi population, has been studied. The formation of biofilms at a certain stage of development of Candida albicans population was revealed.
Keywords
C. albicans, biofilms, electron microscopy, ultrastructure, blastospores, chlamydospores, pseudohyphs
