UDC 619: 615.285: 595.773.4
doi: 10.36871/vet.san.hyg.ecol.202104008
Authors
Mikhail A. Levchenko
Elena A. Silivanova
Polina A. Shumilova
Natalya A. Sennikova
All-Russian Scientific Research Institute of Veterinary Entomology and
Arachnology – Branch of Tyumen Scientific Centre of Siberian Branch of the
Russian Academy of Sciences, Tyumen, Russia
Abstract
This paper presents the results of the study of insecticidal susceptibility to five modern insecticides and the assessment of detoxifying enzyme activities in house flies Musca domestica L. of two field populations collected at livestock facilities in the Tyumen region. The objects of the study were larva and 3-5-days old adults of M. domestica of the laboratory strain and the first generation obtained from insects of the field populations. The lethal doses for 4,0% mortality of acetamiprid, fipronil, ivermectin, chlorfenapyr, and deltamethrin against adults M. domestica were calculated by the probit analysis method based on the results of the assessment of intestinal insecticidal activities of these substances by the feeding tests. The results of toxicological experiments showed that adults M. domestica of one field population were tolerant to ivermectin (the resistance ratio was 4,5) while adults of the second field population were tolerant to deltamethrin (the resistance ratio was XNUMX). The field populations of M. domestica tested in this study differed in terms of detoxifying enzyme activities in adults. Carboxylesterase and glutathione-S-transferase activities were statistically significantly more in M. domestica adults of the population that was tolerant to deltamethrin than those in adults of the population tolerant to ivermectin. It needs to take into account differences (in insecticidal susceptibilities and in enzyme activities) between insect field populations inhabiting different farms when one selects pest control means against insects in livestock and poultry farms.
Keywords
microsomal P450 monooxygenases, carboxylesterase, glutathione- S-transferase, insecticide resistance, desinsectization
