UDC 619.615.33
doi: 10.36871/vet.san.hyg.ecol.202201011
Authors
Victoria B. Muravyeva,
Natalya I. Soboleva,
Oleg A. Makhlis,
Vladimir O. Bondarenko,
All-Russian state center of quality and standardization of medicine for
animal and food, Moscow, Russian Federation
Vasily I. Dorozhkin,
All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology
Branch of Federal State Budget Scientific Institution «Federal Scientific
Center K.I. Skryabin, Ya.R. Kovalenko All-Russian Research
Institute of Experimental Veterinary Medicine, Russian
Academy of Sciences», Moscow,
Russian Federation
Abstract
The article presents the features of conducting experiments on different microbiological media using microbial cultures. Optimal experimental conditions were selected for determining the activity of tylosin in antimicrobial drugs by diffusion into agar. The test microorganism, solvents, buffer solution, nutrient medium and test conditions for achieving a given goal are determined. Determination of the antimicrobial activity of tylosin by the microbiological method is not available in domestic sources. Tylosin is widely used in animal husbandry. As a result of the conducted studies, it was found that the test method for determining the activity of tylosin for work in the laboratory is optimal when the optimal medium for tylosin + M. luteus ATCC 9341 is combined. Laboratories need to determine its antimicrobial activity to perform research in this area. The experimentally obtained method allows us to apply it in the daily routine practice of research laboratories and get the results taken into account.
Keywords
antimicrobial activity, method of diffusion into agar, tylosin
