UDC 619: 614.449.57
doi: 10.36871/vet.san.hyg.ecol.202301007
Authors
Damir I. Udavliev,
Galina V. Filipenkova,
Irina V. Kushch,
Russian Biotechnological University, (BIOTECH University),
Moscow 125080, Russian Federation; All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of
Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko All-Russian
Research Institute of Experimental Veterinary Medicine,
Russian Academy of Sciences,
Moscow 123022, Russian Federation
Anna A. Shustova,
Oleg I. Bashnin,
Sergey S. Shikhov,
Russian Biotechnological University, (BIOTECH University),
Moscow 125080, Russian Federation
Nikolai I. Popov,
All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of
Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko All-Russian
Research Institute of Experimental Veterinary Medicine,
Russian Academy of Sciences,
Moscow 123022, Russian Federation
Julia A. Gulyaeva,
Sibbiopharm, Novosibirsk region, Berdsk 633004, Russian Federation
Asiyat M. Abdullaeva,
Abstract
The article provides information about tests of the preparation containing spores and
cells of the culture-producer Bacillus thuringiensis var. thuringiensis, endotoxin (delta-endotoxin)
and thermostable β-exotoxin. The tests were carried by the method of processing of the tested
drug of organic substrate (manure) in which there were larvae of various insects out in as close
to production conditions as possible in the vivariums of the Institute of Veterinary Medicine,
Veterinary and Sanitary Expertise and Agro-Safety of the Federal State Budgetary Educational
Institution of Higher Education and All-Russian Research Institute of Veterinary Sanitation,
Hygiene and Ecology – branch of the Federal State Budgetary Scientific Research Center of the
Russian Academy of Sciences.
It was found that an average of 10-3 fly larvae of various ages and 15-18 pupae of various
insects were found in a manure substrate with a volume of 6 cm10.The results of semi-reproducible
studies have shown that the preparation when used at a concentration of 3% and a flow rate of 3000-
5000 ml/ m2, reduces the number of flies flying out of the treated substrate by an average of 5,32-6
times during 30 days. The treatment interval was 30 days.
Keywords
endotoxin, thermostable β-exotoxin, Bacillus thuringiensis var. Thuringiensis