SUBSTANTIATION OF THE SENSITIVITY PARAMETERS OF THE BIOLUMINISCENT METHOD FOR THE DETERMINATION OF ANTIBACTERIAL SUBSTANCES IN THE SCREENING ANALYSIS OF FISH PRODUCTS

UDC 619: 614.31: 637.56
doi: 10.36871/vet.san.hyg.ecol.202302010

Authors

Elizaveta A. Denisova,
Veronica S. Babunova,
Galina M. Goryainova,
Luiza.V. Arsenyeva,
All-Russian Research Institute for Veterinary Sanitation, Hygiene and Ecology – Branch of Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences, Moscow 123022, Russian Federation

Abstract

Residual amounts of antibacterial drugs found in fish products and aquaculture can cause significant harm to the consumer, causing allergies and various diseases, so there is a need to develop and validate modern methods that allow not only qualitatively, but quantitatively determine the residual amounts of various antibiotics and antibacterial substances. During the validation of the Randox® Biochip bioluminescent method, we obtained experimental data that showed the promise of this method for detecting residual amounts of antimicrobial substances of various groups in fish and aquatic products. The use of Randox technology significantly reduces the time of analysis of the samples under study, allowing you to simultaneously analyze about 50 samples within one working day. Using the immunomicrochip method, the optimal parameters for sample preparation were determined. The use of the Antimicrobial Array I Ultra panel for the determination of residual amounts of sulfonamides showed the possibility of its use for the simultaneous determination of 20 drugs and sulfonamides.
The use of the Antimicrobial Array II Plus panel made it possible to determine the residual amounts of 35 antibacterial drugs simultaneously from the groups of tetracyclines, thiamphenicols, quinolones, streptomycin, etc. The use of the Antimicrobial Array III panel made it possible to identify residual amounts of several groups of antibacterial drugs simultaneously in one shrimp sample.

Keywords

antibacterial substances, antibiotics, bioluminescent method, immunomicrochip technology, fish, fish products, aquaculture, screening