UDC 619: 614.48
doi: 10.36871/vet.san.hyg.ecol.202302002

Authors

Dmitry V. Gruznov,
Nikolai I. Popov,
Gulizar Sh. Shcherbakova,
Svetlana P. Stepanova,
All-Russian Research Institute for Veterinary Sanitation, Hygiene and Ecology – Branch of Federal Scientific Center – K.I. Skryabin, Ya.R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences, Moscow 123022, Russian Federation
Olga A. Gruznova,
N. N. Semenov Federal Research Center of Chemical Physics of Russian Academy of Sciences, Moscow 119991, Russian Federation
Anton V. Lobanov,
N. N. Semenov Federal Research Center of Chemical Physics of Russian Academy of Sciences, Moscow 119991, Russian Federation; Moscow Pedagogical State University, Moscow 119991, Russian Federation

Abstract

The paper presents the incorporating method of FeIIICl-tetraphenylporphyrin (FeClTPP) into the poly-N-vinylpyrrolidone (PVP) polymeric matrix. The resulting FeClTPP-PVP complex was diluted to final concentrations of 10, 20, 40, 60, 80 and 100 mmol and deposited on the inner surfaces of glass vessels. These vessels were used to simulate the surfaces of objects of veterinary and sanitary control that require periodic disinfection. The antibacterial activity of indicated concentrations of FeClTPP-PVP against antibiotic-resistant gram-negative and gram-positive microorganisms – Escherichia coli (strain 1257) and Staphylococcus aureus (strain 209-P) was demonstrated. As these microorganisms are also representatives of the most frequently encountered contaminants, we have studied the prepared bacterial suspensions from daily cultures of E. coli and S. aureus in dilutions from 108 to 104 CFU/mL. After that, the artificial contamination of the inner surfaces of the test objects was carried out. Antibacterial activity was determined by the degree of inhibition and was recorded visually and spectrophotometrically (OD600 nm ). The inhibitory effect on the growth of E. coli and S. aureus was directly proportional to the concentration of FeClTPP-PVP and inversely proportional to the degree of microorganism’s dilution. The greatest effect (about 87% reduction in bacterial growth) was achieved by the influence of FeClTPP-PVP maximum concentration.

Keywords

disinfectant, FeIIICl-tetraphenylporphyrin (FeClTPP), poly-N-vinylpyrrolidone (PVP), Escherichia coli, Staphylococcus aureus, antibacterial activity.