УДК 619:618.19-002:636.22/28
doi: 10.36871/vet.san.hyg.ecol.202401010

Authors

Evgeniy V. Ivanov,
Andrey V. Kapustin,
Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after K. I. Skryabin and Ya. R. Kovalenko of the Russian Academy of Sciences Moscow 109428, Russian Federation
Natalia N. Avduevskyа,
Vologda branch of Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after K. I. Skryabin and Ya. R. Kovalenko of the Russian Academy of Sciences Vologda 160000, Russian Federation

Abstract

As a result of the conducted studies of 20 milk samples, 84 isolates of bacteria of various species were identified. The incidence of S. aureus isolation was 13,10% (11 isolates), E. coli 14,29% (12 isolates), S. dysgalactiae 9,52% (8 isolates), S. uberis – 8,33% (7 isolates), S. agalactiae and S. pyogenes 7,14% (6 isolates each), K. pneumoniae – 2,38% (2 isolates). The total number of these isolates was equal to 61,9% of the total number of isolated bacteria. Thus, it was established that cow mastitis is caused or complicated by microorganisms of these types of bacteria. An experiment was conducted to establish the effect of immunization of cows with the study of milk samples from animals of the experimental and control groups (200 heads each), as well as in comparison with the microbiome of milk from a group of animals before vaccination (20 heads). As a result, it was found that from the milk of cows of the experimental group, pathogens of infectious mastitis of the listed species are detected 2,4 and 2,5 times less often than in the control and group of animals before vaccination. S. dysgalactiae, S. uberis and K. pneumoniae were not detected in the experimental group, whereas in animals before vaccination and in the control group, their number was 9,52%, 8,33%, 2,38% and 6,97%, 4,65% and 9,30%, respectively. Cultures of S. aureus, S. agalactiae, S. pyogenes and E. coli were isolated in all three groups of animals with the smallest number of isolates of S. aureus, S. agalactiae and E. coli in the experimental group – 1,8, 2 and 2 times less than in the group of animals before vaccination and 1,1, 1,5 and 2,2 times less than in the control group.

Keywords

infectious mastitis, milk microbiome, immunity, vaccine against mastitis