SPECTRA OF Fragments of Genomic DNA Flanked by Microsatellites and Endogenous Retroviruses in Mouflon and Aboriginal Sheep Breeds

DOI: 10.26155 / VET.ZOO.BIO.201901013
UDC 636.2: 577.212.3

Authors

V. I. GLAZKO, G. Yu. KOSOVSKY, I. I. GAPONOV, T. A. ERKENOV, R. A. KRYUKOVSKY, T. T. GLAZKO
MOSCOW AGRICULTURAL ACADEMY NAMED AFTER K.A. TIMIRYAZEV

Abstract

The genetic relationships between wild and domestic representatives of the Ovis genus, the European mouflon (Ovis orientalis musimon) and domestic sheep (Ovis aries), are examined. As a result of multilocus genotyping using ISSR-PCR and IRAP-PCR markers, data were obtained indicating that mouflons are more genetically heterogeneous compared to native sheep breeds both from DNA fragments flanked by inverted repeats of microsatellites and from fragments of long terminal repeats of endogenous retroviruses . When using IRAP-PCR and ISSR-PCR markers, mouflons are included in the same cluster with sheep of steppe breeds, such as Edilbaevskaya and Kalmyk. The most consolidated are the Edilbaev and Kalmyk sheep breeds. The Karachaevskaya sheep breed stands out in an autonomous cluster, which may indicate the specificity of its formation, as well as adaptation to the conditions of the highlands.

Keywords

genomic scanning, mouflon, sheep, ISSR-PCR, IRAP-PCR.