ANTIBACTERIAL EFFECTS OF BAS OF DIFFERENT ORIGIN AND THEIR COMBINED ACTION WITH SOME? -LACTAMIC ANTIBIOTICS

DOI: 10.26155 / VET.ZOO.BIO.201901008
UDC 577.181.5

Authors

I. A. GNEUSHEVA, N. E. PAVLOVSKAYA, A. V. LUSHNIKOV
ORLOV STATE AGRARIAN UNIVERSITY NAMED AFTER N.V. PARAKHIN

Abstract

We studied the antibacterial action of some biologically active substances of plant and microbial origin, as well as their combinations with? -Lactam antibiotics in relation to clinical isolates of gram-negative bacteria Escherichia coli. Low molecular weight exometabolites of Trichoderma harzianum (23–30 mm) exhibit antagonistic activity. The buckwheat bioflavonoid extract is characterized by moderate antibacterial effects (9–19 mm). Growth characteristics of bacteria positively correlated with the effects of biologically active substances and their components in bacteriostatic concentrations of 3,9 μl / ml (dilution 1: 256) and 1,95 μl / ml (dilution 1: 512) for plant metabolites; at bactericidal concentrations of 0,97 μl / ml (dilution 1: 1024), 0,48 μl / ml (dilution 1: 2048) for microbial. Responsive to the combined effect with exometabolites of Trichoderma harzianum and their active component? -Phenylethanol? -Lactam antibiotics cefuroxime, meropenem and autronam. Synergistic compositions based on them can be promising for the creation of complex antimicrobial agents for use in veterinary medicine.

Keywords

antibacterial activity, plant and microbial biologically active substances, Trichoderma harzianum exometabolites, extract of bioflavonoids from buckwheat flowers,? -lactam antibiotics, synergistic compositions.