DOI: 10.26155 / VET.ZOO.BIO.201902005
UDC 612.12

Authors

O. A. VORONINA
MOSCOW STATE ACADEMY OF VETERINARY MEDICINE AND BIOTECHNOLOGY - MBA NAMED AFTER K. I. SKRYABIN
L. A. Ilyina
LLC BIOTROF +
S. Yu. ZAITSEV
MOSCOW STATE ACADEMY OF VETERINARY MEDICINE AND BIOTECHNOLOGY - MBA NAMED AFTER K. I. SKRYABIN

Abstract

The state of health of lactating cows largely depends on the functioning of the enzyme systems of the body. It is the level of enzymatic activity of blood serum, determined in a biochemical analysis, that indicates to a specialist the intensity of biochemical processes and the state of health of the animal. The balance of the synthesis rate of enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), amylase, alkaline phosphatase (ALP), lactate dehydrogenase (LDH) inside the cells and their exit from the cells is easy to track by their level in blood serum. It should be borne in mind that the enzymatic activity of blood serum of cows may differ depending on the period of lactation. Along with traditional methods of biochemical analysis, which directly express the activity of enzymes, in our work we used a modern analytical method for determining the parameters of dynamic surface tension (DPN). The set of data on the biochemical and DPN parameters of blood in cattle and lactating cows (in our current and previous works) is of both fundamental and practical value for veterinary, zootechnical and biological sciences. Earlier, we demonstrated a correlation between the content of various fractions of protein and lipids with a number of DPN parameters. In this work, a strong positive correlation was established between parameter α3 and serum alkaline phosphatase activity.

Keywords

biochemical analysis, enzymatic activity, dynamic surface tension, lactation, cattle, metabolism.