DOI: 10.26155 / VET.ZOO.BIO.201903013
UDC 619
Authors
A. A. Savina, I. S. Zaitsev, A. M. Barvinsky, M. R. Gilfanova, S. Yu. Zaitsev
MOSCOW STATE ACADEMY OF VETERINARY MEDICINE AND BIOTECHNOLOGY - MBA NAMED AFTER K. I. SKRYABIN
Abstract
One of the priority areas of actively developing bionanotechnology is the creation of functional preparations based on enzymes. In particular, enzyme immobilization methods (including lipases) are comprehensively studied to create biocomposites with a high degree of activity and stability preservation. The purpose of this review article was to examine in detail the features, advantages, and disadvantages of immobilizing lipases (from various sources) on nanoscale carriers, such as particles of metals and their oxides. According to published data, the immobilization of lipases on nanoscale carriers is a promising direction in the creation of bio-nanocomposites with sufficient activity and stability. However, the considered methods (both during adsorption and covalent immobilization) describe a partial inhibition of the enzymatic activity of the obtained lipolytic drugs. At the same time, the key advantage of immobilization is the prolonged action of the enzyme in the drug, increasing its thermal stability and resistance to denaturing factors. Immobilization of lipase is not only economically more beneficial for a number of applications, but also simplifies the control of the production process and allows multiple use of both the carrier and the enzyme preparation. This approach, applied with respect to lipases, can help in solving problems such as the synthesis of new biological products, the disposal of oil processing products, the delivery of various biomolecules and their controlled release in a given organ, the construction of new bio-nanocomposites, etc.
Keywords
biochemical analysis, enzymatic activity, lipases, gold nanoparticles, silver nanoparticles, magnetite.
