DOI: 10.26155 / VET.ZOO.BIO.201903004
UDC 547.262: 547.281.2: 591.111
Authors
I.P. STEPANOVA, A.G. PATYUKOV, T.V. POSTNOVA, Y. S. MAKAROV L.I. SUKACH
OMS STATE MEDICAL UNIVERSITY OF THE MINISTRY OF HEALTH OF THE RUSSIAN FEDERATION
Abstract
At present, the waste of brewing (brewer's grains) and alcohol (spirit distillery) industries are added to the feed of farm animals and birds. These concentrates enhance the fermentation processes in the rumen of ruminants, which can lead to an increase in the physiological level of ethanol and acetaldehyde in the tissues and have a toxic effect on the body. The level of ethanol and acetaldehyde in the blood and tissues of mammalian animals depends on many factors, in particular on the various phases of the reproductive cycle, the feeding system, and external environmental influences. The endogenous and exogenous levels of these substances in the blood and tissues of monogastric mammals are well studied, while similar studies have not been conducted in polygastric (in particular cattle), and information on the content of ethyl alcohol and acetaldehyde in the blood of these animals is not available. The aim of the study was to study the concentration of endogenous ethanol, acetaldehyde, enzymes that reflect the functional state of the liver (AlAT, AsAT, GGT, ALP) and indicators of carbohydrate (glucose) and lipid (triacylglycerides) metabolism of cows in various physiological conditions to develop new biochemical blood tests. The object of the study was cows of black-motley breed aged 3-4 years with similar indicators of live weight and milk productivity. The following groups of animals were studied (n = 10): the 15th day of dead wood (1st group), the 7–10th day before calving (2nd group), the 15th day after calving (3rd group) and 3-4 months after calving (4th group - control). The studies were performed by capillary gas chromatography. The concentration of endogenous ethyl alcohol in the blood of cows is approximately at the same level during the period of deep pregnancy, pre- and post-hotel periods, while 3-4 months after calving, there is a tendency to its decrease. The content of acetaldehyde is approximately at the same level in cows under various physiological conditions and is almost 10 times lower than the amount of endogenous ethyl alcohol. Moreover, the concentration of acetaldehyde is more stable than the level of ethanol. Biochemical blood parameters of animals characterizing the functional state of the liver and kidneys are within the physiological norm. The results obtained are recommended for use in clinical diagnostics to control the level of endogenous acetaldehyde and ethanol, and study their metabolism.
Keywords
endogenous ethanol, acetaldehyde, cattle.
