CULTIVATION OF VIRUS PARAGRIP-3 OF CATTLE IN CULTURE OF CELLS RECEIVED FROM MOUSE KIDNEY TISSUE

DOI: 10.26155 / VET.ZOO.BIO.201903008
UDC 619.616.98: 578.835.2-085.37

Authors

I.V. TRETYAKOVA, M.S. KALMYKOVA, E. I. YARYGINA
MOSCOW STATE ACADEMY OF VETERINARY MEDICINE AND BIOTECHNOLOGY - MBA NAMED AFTER K. I. SKRYABIN

Abstract

The work is devoted to the study of the sensitivity of cell culture obtained from kidney tissue of a newborn mouse to the parainfluenza-3 virus of cattle (cattle). According to the results of the work, the authors found that in the mouse kidney cell culture, which gives a stable fibroblast-like cell line, cattle parainfluenza-3 virus is cultivated with an infectious titer of at least 104,0 TCD50 / ml. It was shown that this cell culture is not inferior in sensitivity to cattle parainfluenza-3 virus to such transplantable cell cultures as calf kidney (PT) and cow lung embryo (LEC).

Keywords

mouse kidney cell culture, calf kidney cell culture, cow embryonic lung cell culture, green monkey kidney cell culture, cytopathic effect, infectious activity, effective dose, cytopathic effect, cell monolayer, light microscopy, titration.