MODIFICATION OF THE METHOD FOR DETERMINING THE ACETYLING ABILITY OF ANIMAL ORGANISM

DOI: 10.26155 / VET.ZOO.BIO.201905005
UDC 619: 57.088.6: 636.2

Authors

Yu. N. ALEKHIN, T. D. WEALTH
ALL-RUSSIAN RESEARCH INSTITUTE OF PATHOLOGY, PHARMACOLOGY AND THERAPY, G. VORONEZH, RUSSIAN FEDERATION

Abstract

In the present work, the results of evaluating the analytical reliability of the method for determining the acylating ability of an organism according to the Bratton-Marshall method in the modification of L. N. Bulovskaya, G. N. Borisenko and L. M. Kosenko are presented. The risk points of the technology for performing this method were identified and options for reducing their severity were proposed. To reduce the likelihood of an analytical error, the concentration of the indicator (N- (1) -naphthylethylenediamine dihydrochloride) was increased from 0,05 to 0,1%, the hydrochloric acid solution was not added to the contents of the tubes at the same time, to the hydrolyzed sample before boiling, and to the non-hydrolyzed sample before setting her in the fridge. The changes made it possible to increase the analytical reliability of the method for determining acetylating ability, reducing the variability of photocolorimetric studies and by 54,7% of animal indicators by 48,9%.

Keywords

calves, piglets, acetylating ability of the body, sulfonamide, analytical reliability.