DOI: 10.36871/vet.zoo.bio.202101004
UDC 619: 615.9

Authors

A. N. Shkatova
Postgraduate Student of the Department of Immunology and Biotechnology, Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K.I. Skryabin, Moscow, Russian Federation
D. A. Devrishov
Doctor of Biological Sciences, Professor, Corresponding Member of the Russian Academy of Sciences, Head of the Department of Immunology and Biotechnology, Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K.I. Skryabin, Moscow, Russian Federation
O. B. Litvinov
Doctor of Veterinary Sciences, Professor, Department of Immunology and Biotechnology, Moscow State Academy of Veterinarian Medicine and Biotechnology – MVA by K. I. Skryabin, Moscow, Russian Federation
V. E. Brylina
Candidate of Veterinary Sciences, Associate Professor of the Department of Immunology and Biotechnology, Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K.I. Skryabin, Moscow, Russian Federation

Abstract

In the course of the studies, the toxicological properties of the medicinal product for veterinary «Ketoprofen 10%» in laboratory animals were studied.
With a single intragastric administration of the drug to rats, the LD50 value calculated by the Kerberian method was 333,3 mg/kg. At the same time, the LD50 value by the Miller and Tainter method was 358,1±159,8 (198,3 ÷ 518,0) mg/kg.
After a single intravenous injection of the study drug to rats, the LD50 value calculated by the Kerberian method was 351,1 mg/kg. The LD50 calculated by probit analysis according to Miller and Tainter was 349,7±167,5 (182,3 ÷ 517,2) mg/kg.
Taking into account the obtained LD50 value in rats with intragastric administration and according to the generally accepted hygienic classification, the drug belongs to the 3rd hazard class – moderately hazardous substances (GOST 12.1.007-76).
Intragastric administration of the drug to mice made it possible to obtain an LD50 value of 6249,4 mg/kg (according to Kerber). The LD50 calculated by probit analysis according to Miller and Tainter was 7725,0±2646,5 (5078,5 ÷ 10) mg/kg.
Taking into account the data obtained on mice and according to the generally accepted hygienic classification, the drug belongs to the 4th hazard class – low-hazard substances (GOST 12.1.007-76).
The coefficient of species sensitivity was more than 17, which indicates a pronounced species resistance to ketoprofen in mice compared to rats.

Keywords

ketoprofen, acute toxicity, intragastric administration, intravenous administration, mice, rats, LD50 , body weight, hematology, biochemistry.