DOI: 10.36871/vet.zoo.bio.202104010
UDC 547.318: 541.64
Authors
I. V. Milaeva
Candidate of Biological Sciences, Associate Professor,
Associate Professor of the Department of Chemistry
named after Professors S. I. Afonsky, A. G. Malakhov,
Moscow State Academy of Veterinary Medicine
and Biotechnology – MVA by K. I. Skryabin,
Moscow, Russian Federation
S. Yu. Zaytsev
Doctor of Biological Sciences, Doctor of Chemical Sciences, Professor,
Leading Researcher, Head of the Analytical Biochemistry Group
of the Department of Physiology and Biochemistry of Farm
Animals, All-Russia Research Institute of Animal
Husbandry named after Academy Member L. K. Ernst,
Dubrovitsy, Russian Federation
Abstract
Due to the significant increase in the resistance of microorganisms to the action of medicines,
primarily antibiotics, the search for other antibacterial agents, including the preparation of
silver-based drugs, is extremely important. In small concentrations (50 mcg/l), silver is safe for a
living organism, but shows a destructive effect against most bacteria and viruses. The use of silver
in the form of nanoparticles (NPAg) can reduce its concentration by hundreds of times compared
to the ionic form, while preserving all its antimicrobial properties. An important task is both the
development of non-toxic antimicrobial drugs based on silver NP that retain their bactericidal properties
and stability over a long period, and the elucidation of the mechanisms of interaction of NPAg
with cells and tissues of a living organism.
The aim of this work was to study the effect of specified concentrations of NPAg on the structure
(size) of particles and surface tension (ST) of model systems obtained on the basis of crude egg phosphatidylcholine
and cholesterol. In the work, mixtures of the following lipids were studied in detail:
PCe1 – crude phosphatidylcholine, Chol – cholesterol. It was shown that under the influence of
middle concentrations of NPAg (up to 0,3–0,5 rel. units) in samples with PCe1 significant changes
in the particle size in the mixture occur. Moreover, in mixtures of Chol with PCe1, the addition of
NPAg even at a low concentration (0,01 rel. units) already causes a decrease in the average particle
diameter by 1,5 times. It has been shown that the presence of sufficiently large NPAg particles (at
surface lifetimes from 0,5 to 5 s) gives higher ST values, and a change in the particle size leads to
changes in ST: an increase in size – an increase in ST, a decrease in size – a decrease in ST. The equilibrium ST is apparently influenced by the presence of NPAg particles of different sizes in the
sample and their ratio.
A study of the interaction of NPAg with natural compounds, such as phospholipids, polysaccharides,
proteins and peptides, is of great fundamental and practical importance.
Keywords
lipids, phosphatidylcholine, cholesterol, silver nanoparticles, surface tension of mixtures.
