DOI: 10.36871/vet.zoo.bio.202105012
UDC 612.12: 577.16 + 612.661.5: 577.16
Authors
A. A. Savina
Junior Researcher, Department of Physiology and Biochemistry of Farm Animals
of the L. K. Ernst Federal Research Center for Animal Husbandry,
Dubrovitsy, Russian Federation
O. A. Voronina
Candidate of Sciences in Biology, Veterinarian, Senior Researcher of the Department of
Physiology and Biochemistry of Agricultural Animals of the L. K. Ernst
Federal Research Center for Animal Husbandry,
Dubrovitsy, Russian Federation
L. P. Ignatieva
Candidate of Agricultural Sciences, Leading Researcher of the Department
of Population Genetics and the Genetic Basis of Animal Breeding of
the L. K. Ernst Federal Research Center for Animal Husbandry,
Dubrovitsy, Russian Federation
N. V. Bogolyubova
Candidate of Biological Sciences, Head of the Department of Physiology
and Biochemistry of Farm Animals of the L. K. Ernst
Federal Research Center for Animal Husbandry,
Dubrovitsy, Russian Federation
S. Yu. Zaytsev
Doctor of Biological Sciences, Doctor of Chemistry, Professor, Leading Researcher
at the Department of Physiology and Biochemistry of Farm Animals of
the L. K. Ernst Federal Research Center for Animal Husbandry,
Dubrovitsy, Russian Federation
Abstract
The aim of the work was to study the component composition of milk of black-and-white cows in
the winter-stall period and its main biochemical parameters. In this work, the total amount of
water-soluble antioxidants (TAWSA) was assessed by the amperometric method (using "TsvetYauza
01-AA" device) and the average values of the milk component composition for four groups of
cows, which were formed by the number of somatic cells (NSC) found in their milk. Milk of mastitis
cows causes food poisoning that is why 1 groups were formed according to the NSC values: 200) less
than 2 thousand units/ml, 200) from 499 to 3 thousand units/ml, 500) from 999 to 4 thousand
units/ml and 1000) more than XNUMX thousand units/ml for milk samples of black-and-white cows of the
Moscow region, which was used as raw material.
The TAWSA values for 33 milk samples from cows in group 1 ranged from 6,80 to 27,91 mg/g
(mean value 15,95±3,70 mg/g). The correlations between TAWSA and the following parameters of
milk: mass fraction of fat (MFF) – 0,305; protein mass fraction true (PMF1) and total (PMF2) – 0,197
and 0,210; lactose – 0,156; dry fat-free milk residue (FFMR) – 0,276; total dry matter (TDM) – 0,399;
freezing point (FP) – 0,112; pH – 0,114; the number of somatic cells (NSC) – (–0,052) are found. The
TAWSA values for 15 milk samples from cows in group 2 ranged from 10,46 to 18,99 mg/g (average
value 14,45±3,50 mg/g) are found. The correlations between TAWSA and the following parameters
of milk: MFF – 0,332; PMF1 and PMF2 – 0,296 and 0,303; lactose – (–0,308); FFMR – 0,159;
TDM – 0,391; FP – 0,226; pH – (–0,211); NSC – 0,193. The TAWSA values for 13 milk samples of
cows in group 3 ranged from 10,46 to 18,99 mg/g (average value 16,04±3,60 mg/g) are found. The
correlations between TAWSA and the following parameters of milk: MFF – (–0,352); PMF1 and
PMF2 – (– 0,411) and (–0,401); lactose – (–0,166); FFMR – (–0,462); TDM – (–0,504); FP – (–0,766);
pH – (–0,047); NSC – (–0,698). The TAWSA values for 12 milk samples from cows in group 4 ranged
from 5,80 mg/g to 20,30 mg/g (average value 14,58±3,50 mg/g) are found. The correlations between
TAWSA and the following parameters of milk: MFF – 0,159; PMF1 and PMF2 – 0,046 and 0,077;
lactose – (–0,317); FFMR – (–0,237); TDM – 0,058; FP – (–0,036); pH – (–0,477); NSC – (–0,072) are
found. These data obtained are of great importance both in assessing the physiological-biochemical
status and the state of the antioxidant defense system of the cows' organism.
Keywords
analytical biochemistry, amperometric detection, milk composition, antioxidant protection, amino acids, blood serum.
